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ENVIROLOGIX Inc. - Environmental Mycotoxins - Molds

TRICHOTHECENES - QuantiTox™ (ELISA Kit)

 Intended Use 

The EnviroLogix QuantiTox Kit For Trichothecenes Is A 96-well Plate Kit Designed For The Quantitative Laboratory Detection Of Tricothecenes, Including Roridin A, E, H and L-2, Satratoxin G And H, Isosatratoxin F, Verrucarin A And J, And Verrucarol In Bulk Samples.  The Assay’s Quantitation Range Is From 0.2 To 18.0 Parts Per Billion (ppb) Of Roridin A In The Sample Extract.

General

 

This Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, tricothecenes in the sample compete with enzyme (horseradish peroxidase) labeled satratoxin for a limited number of antibody binding sites on the inside surface of the test wells.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker color = Lower concentration

Lighter color = Higher concentration

Materials Provided: Materials Required But Not Provided:
  • 8 x
    		• 12 antibody-coated strips of wells, in plate frame.
  • 1 x
    		•  Roridin A Stock Solution in 50% MeOH (900 ppb).
  • 1 x
    		• Satratoxin Enzyme Conjugate
  • 1 x
    		•  Substrate.
  • 1 x
    		• Stop Solution.
  • 1 x
    		•  Packet of PBS salts (Sigma Chemical, Cat. # P-3813).
  		• Disposable tip, adjustable air-displacement pipette to deliver 100 µL - 1000 µL (optional).
  		• Positive displacement pipette to deliver 10, 20 and 100 µL (Microanatomy or equivalent).
  		• Twelve-channel pipette to deliver 50 µL and 100 µL (optional).
  		• Marking pen (indelible).
  		• Tape or Parafilm.
  		• Timer.
  		• Microtiter plate reader or strip reader.
  		• Microtiter plate washer (optional).
  		• Racked dilution tubes for loading samples into the plate with a 12-channel pipette (optional).
  		• Distilled or deionized water.
  		• Orbital plate shaker (optional).

     

    Kit Specifications:
    Format: 
    • 96 well.
    Time Requirement: 
    • Results in 1 hour.
    Detection Limit:
    • 0.14 ppb.
    Measurement: 
    • Microtiter Plate Reader.
    Product ID #:
    • EP100.