| R-BIOPHARM
- Enzymatic Bio Analysis and Food Analysis
RAFFINOSE
(UV Test)
|  |
Intended Use
|
UV Test
For The Determination Of Raffinose And D-Galactose
In Sugar Refinery Products, In Soy Bean And Cereal Flour
As Well As In Biological Samples.
| |
General |
|
Raffinose is a tri-saccharide
consisting of D-galactose, D-glucose and D-fructose.
Raffinose is contained in sugar beet in significant
quantities, especially in beets which have been stored
at prolonged cold temperature. In sugar production,
raffinose accumulates in the molasses and has a negative
influence on the crystallization of sucrose. Raffinose
is also contained in other plants, e.g. in soybean.
Its presence in food is indirect proof of adulteration
by the unallowed addition of soybean products.
| Materials
Provided: |
|
1 x |
Lyophilizate
(320 mg); consisting of: citrate buffer (pH approx.
4.5); NAD (approx. 28mg). |
|
1 x |
œ-Galactosidase
suspension (approx. 36 U); 1.6 ml. |
|
1 x |
Potassium
diphosphate buffer solution (34 ml); pH approx.
8.6. |
|
1 x |
œ-Galactose
dehydrogenase suspension (approx. 30 U); 1.6 ml. |
| Kit
Specifications: |
| Detection
Limit: |
|
| Specificity:
|
- Besides
raffinose, œ-galactosidase also hydrolyzes
other œ-galactosides, e.g. galactinol, melibiose
and stachyose. Substances with œ-galactoside
linkages, such as lactose are not hydrolyzed
and do not interfere with the assay, even when
present in high concentrations. Apart from D-galactose,
Gal-DH also oxidizes L-arabinose. Small amounts
of L-arabinose only occur in foodstuffs, if
it has been released from its natural glycosidic
linkage due to chemical and enzymatic influences.
In the analysis of commercial raffinose, results
of approx. 99 % have to be expected.
|
| Linearity: |
-
Linearity of the determination exists from 3
mg raffinose + D-galactose/assay (5 mg raffinose
+ D-galactose/l sample solution; v = 0.500 ml)
to 250 mg raffinose + D-galactose/assay (2.5
g raffinose + D-galactose/l sample solution;
v = 0.100 ml).
|
| Precision: |
-
In a double determination of D-galactose using
one sample solution, a difference of 0.005 to
0.010 absorbance units may occur; in a double
determination of raffinose (in the presence
of D-galactose in the sample) a difference of
0.010 to 0.015.
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| Samples
Per Kit: |
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| Product
ID #: |
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