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ENVIROLOGIX Inc. - Human Exposure Monitoring Kits

METOLACHLOR MERCAPTURATE (ELISA Kit)

 Intended Use 

The EnviroLogix Metolachlor Mercapturate In Urine Plate Kit Is Designed For The Quantitative Laboratory Detection Of Metolachlor Mercapturate Residues In Human Urine Samples, With A Quantitative Assay Range From 8 To 85 Parts Per Billion (ppb).

General

The EnviroLogix Metolachlor Mercapturate in Urine Plate Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, Metolachlor Mercapturate residues in the sample compete with an enzyme (horseradish peroxidase) labeled Metolachlor Mercapturate analogue for a limited number of antibody binding sites on the inside surface of the test wells.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker color = Lower concentration

Lighter color = Higher concentration

The EnviroLogix Metolachlor Mercapturate in Urine Plate Kit can be used in the following testing applications:

  • Human Exposure Monitoring (Applicators, Handlers, Farm Workers).
  • Public Health Screening Programs.
  • Health Risk Assessment.

Materials Provided: Materials Required But Not Provided:
  • 8 x
    		•  Strips of 12 Antibody-Coated Wells each, in plate frame.
  • 1 x
    		•  Vial of Negative Control.
  • 1 x
    		• Vial of 8 ppb Metolachlor Mercapturate Calibrator (Actual Concentration = 0.16 ppb*)
  • 1 x
    		•  Vial of 25 ppb Metolachlor Mercapturate Calibrator (Actual Concentration = 0.5 ppb*).
  • 1 x
    		• Vial of 85 ppb Metolachlor Mercapturate Calibrator (Actual Concentration = 1.7 ppb*).
  • 1 x
    		•  Bottle of Enzyme Conjugate.
  • 1 x
    		•  Bottle of 10x Sample Diluent.
  • 1 x
    		•  Bottle of Substrate.
  • 1 x
    		•  Bottle of Stop Solution.
    * The difference between the stated and actual concentration is to correct for the 1:50 dilution required for sample preparation.

  • Disposable Tip, Adjustable Air Displacement Pipette which will measure 980 microliters (µL), 100 µL, and 20 µL.

  • Glass Test Tubes.

  • Marking Pen (indelible).

  • Tape or Parafilm.

  • Timer (1 hour and 30 minutes).

  • Distilled Water.

  • Cool Tap Or Distilled Water for rinsing wells.

  • Microtiter Plate Reader or strip reader.
  		• Microtiter Plate Washer (optional).
  		• Twelve-Channel Pipette that will measure 100 µL (optional).
  		• Racked Dilution Tubes for loading samples into the plate with a 12-channel pipette (optional).
  		• Orbital Plate Shaker (optional).

    Kit Specifications:
    Plate Format: 
    • 96 Well.
    Time Requirement: 
    • 2 h 15 min.
    Detection Limit:
    • 5 ppb.
    Quantification Limit:     
    • 10 ppb.
    Cross Reaction:
    (Limit Of Detection)
    • Metolachlor Mercapturate (5 ppb).
    • Alachlor Mercapturate (63 ppb)
    • Atrazine Mercapturate (510000 ppb).
    • Acetochlor Mercapturate (175 ppb).
    • Metolachlor ESA (40 ppb).
    • Alachlor ESA (915 ppb).
    • Acetochlor ESA (9050 ppb).
    Measurement: 
    • Microtiter Plate Reader or Strip Reader.
    Product ID #:
    • BP002.