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ENVIROLOGIX Inc. - Environmental Monitoring Kits - Natural Toxins

MICROCYSTIN - QuickTube™ Kit (ELISA Kit)

 Intended Use 

The EnviroLogix Microcystin Tube Kit Is Designed For Semi-Quantitative Field Screening Of Microcystin Toxin In Surface Water Samples. The Kit Is Supplied With Calibrators At 0.5 And 3 Ppb. The Assay Range Can Easily Be Extended.

General

The EnviroLogix Microcystin Tube Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, Microcystin toxin in the sample competes with enzyme (horseradish peroxidase) labeled Microcystin for a limited number of antibody binding sites on the inside surface of the test tubes.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker color = Lower concentration

Lighter color = Higher concentration

The EnviroLogix Microcystin Tube Kit can be used in the following testing applications:

  • Early Warning Of Bloom Formation And Water Quality Degradation.
  • On-Site Ground And Surface Water Screening And Monitoring.
  • Ensure Guidance Levels Are Not Exceeded In Drinking Water, Surface Water, And Algae Based Food Supplements.
  • Quality Control Testing Of Raw Material And Finished Algae Based Products.

Materials Provided: Materials Required But Not Provided:
  • 36 x   Antibody-Coated Test Tubes
  • 1 x   Vial of 0.5 ppb Microcystin LR Calibrator (detoxified).
  • 1 x   Vial of 3 ppb Microcystin LR Calibrator (detoxified).
  • 1 x   Dropper Bottle of Assay Diluent.
  • 1 x   Dropper Bottle of Microcystinenzyme Conjugate
  • 1 x   Dropper Bottle of Substrate.
  • 1 x   Bottle of Stop Solution.
  • 36 x   Sample Pipettes.
  • Marking Pen (indelible).
  • Timer (5, 20 and 10 minutes).
  • Cool Tap Or Distilled Water for rinsing tubes, in a wash bottle.
  • Photometer for reading tubes (optional).
  • Test Tube Rack that can hold at least 6 tubes securely enough to flick out.
  • Water.
  • After Wash Step (Contact Xygen for information on obtaining an appropriate rack).
  • Disposable Tip, Adjustable Air-Displacement Pipette which will measure 0.7 mL (optional).

    Kit Specifications:
    Plate Format: 
    • 36 Test Tubes.
    Time Requirement: 
    • 1 h 10 min.
    Detection Limit:
    • 0.3 ppb.
    Cross Reaction:
    (Limit Of Detection)
    • Microcystin LR (0.30 ppb).
    • Microcystin LA (0.43 ppb).
    • Microcystin RR (0.65 ppb).
    • Microcystin YR (0.69 ppb).
    • Nodularin (0.53 ppb).
    Measurement: 
    • Visual or with Microtiter Plate Reader.
    Product ID #:
    • ET022.