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ENVIROLOGIX
Inc. - GMO Monitoring Kits
CRY1Ab/CRY1Ac
Plate Kit (Sandwich ELISA Kit)
|  |
Intended Use |
The EnviroLogix
Cry1Ab/Cry1Ac Plate Kit Is Designed For The Quantitative
Laboratory Detection Of Cry1Ab Or Cry1Ac Residues In
Corn And Cotton Leaf Tissue Samples.
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General
Use |
|
The
EnviroLogix Cry1Ab/Cry1Ac Plate Kit is a “sandwich”
Enzyme-Linked ImmunoSorbent Assay (ELISA). In the test
plant leaf sample extracts are added to test wells coated
with antibodies raised against Cry1Ab toxin. Any residues
present in the sample extract bind to the antibodies
and are then detected by addition of the enzyme (horseradish
peroxidase) labeled Cry1Ab antibody. After a simple
wash step the results of the assay are visualized with
a color development step; color development is proportional
to Cry1Ab/Cry1Ac concentration in the sample extract.
Lighter
color = Lower concentration
Darker
color = Higher concentration
The EnviroLogix Cry1Ab/Cry1Ac Plate Kit Can Be Used
In The Following Testing Applications:
- Quality Assurance During Transgenic Seed Production.
-
Large Scale Screening Of Plants To Assess Expression
Of Gene.
- Checking Truckloads Or Containers Of Grain At Elevator,
Barge, Or Processing Plant.
| Materials
Provided: |
Materials Required
But Not Provided: |
|
12 x |
Strips
Of 8 Antibody-Coated Wells each, in plate
frame. |
|
1 x |
Vial
of Cry1Ab Negative Control. |
|
1 x |
Vial
of 0.5 ppb Cry1Ab Calibrator.
|
|
1 x |
Vial
of 2.5 ppb Cry1Ab Calibrator. |
|
1 x |
Vial
of 5 ppb Cry1Ab Calibrator. |
|
1 x |
Bottle of Cry1Ab-Enzyme Conjugate. |
|
1 x |
Bottle of 5x Cry1Ab Extraction/Dilution Buffer
|
|
1 x |
Packet Of Wash Buffer Salts |
|
1 x |
Bottle Of Substrate. |
|
1 x |
Bottle
Of Stop Solution. |
|
1 x |
Bottle
20x Grain Extraction. |
|
1 x |
Concentrate
(for use with attached Application Guide). |
|
|
Disposable
Tissue Extractors, EnviroLogix Cat. # ACC
002.
|
|
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Test
Tubes for dilution of sample extracts.
|
|
Disposable Tip, Adjustable Air-Displacement
Pipettes which will measure 20, 100, 500 and
1000 |
|
|
Microliters
(µL). |
|
Marking
Pen (indelible). |
|
|
Tape
or Parafilm. |
|
Timer
(15 minutes, 1 hour, and 30 minutes). |
|
|
Distilled
Or Deionized Water for preparing Wash Buffer
and diluting 5x Cry1Ab Extraction/Dilution. |
|
Buffer. |
|
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Glass
Bottles or flasks with 175 mL capacity for
storage of 1x Cry1Ab. |
|
Extraction/Dilution
Buffer and 1 liter capacity for Wash Buffer. |
|
|
Microtiter Plate Reader or strip reader. |
|
Wash Bottle, or microtiter plate or strip
washer. |
|
|
Multi-Channel
Pipette that will measure 100 µL (optional). |
|
Racked Dilution Tubes for loading samples
into the plate with a multichannel pipette
(optional). |
|
|
Orbital
Plate Shaker (optional). |
|
| Kit
Specifications: |
| Plate
Format: |
|
| Time
Requirement: |
|
| Detection
Limit: |
|
Cross
Reaction:
(Limit
of Detection) |
-
Cry1Ab (0.14 ppb).
- Cry1Ac
(1.2 ppb).
-
Cry1C (31.5 ppb).
-
Cry1F (7 ppb).
-
CryIIA (>10000 ppb).
|
| Measurement:
|
- Microtiter
Plate Reader or Strip Reader.
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| Product
ID #: |
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