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ENVIROLOGIX Inc. - GMO Monitoring Kits

CRY1Ab/CRY1Ac - QuickstixTM For Tissue And Seed

 Intended Use 


The EnviroLogix QuickStixTM Kit For Cry1Ab/Ac Leaf And Seed Is Designed To Extract And Detect The Presence Of The Cry1Ab And Cry1Ac Bt Endotoxins At The Levels Typically Expressed In Genetically Modified Corn And Cotton Plant Tissue.



General Use

 

Corn and cotton crops that have been genetically modified with a Bt gene express Bt endotoxins in their tissue.  To detect these Cry1Ab and Cry1Ac proteins with the EnviroLogix Cry1Ab/Ac QuickStix Strip, tissue samples must be extracted and the endotoxins solubilized in the Extraction Buffer provided.  Each QuickStix Strip has an absorbent pad at each end.  The protective tape with the arrow indicates the end of the strip to insert into the extraction tube.  The sample will travel up the membrane strip and be absorbed into the larger pad at the top of the strip.  The portion of the strip between the protective tape and the absorbent pad at the top of the strip is used to view the reactions as described under “Interpreting the Results.”

The EnviroLogix Cry1Ab/Cry1Ac QuickStix Kit can be used in the following testing applications:

  • Quality Assurance during transgenic seed production.
  • Large scale affordable screening of plants to assess expression of gene and presence of desired attribute.
  • Checking grain for acceptance at elevators and processing plants.
  • Remote testing at international, export or import locations.

Materials Provided:
  •  100 x
  • QuickStix strips packed in two moisture resistant canisters.
  •  100 x
  •  
    Disposable Tissue Extractors (each consisting of a tube and pestle, with punch cap).
  •     4 x
  •  
    Bottles (16 mL each) of Extraction Buffer.

    Kit Specifications:
    Format: 
    • 100 strips.
    Time Requirement: 
    • 10 min.
    Detection Limit:
    • 0.14 ppb.
    Cross Reaction:
    (Limit of Detection)
    • Cry1Ab (10 ppb).
    • Cry1Ac (10 ppb).
    • Cry1C (100 ppb).
    • Cry1F (100 ppb).
    Measurement: 
    • Visual.
    Product ID #:
    • AS003LS.