ENVIROLOGIX
Inc. - GMO Monitoring Kits
CRY1Ab
QUALIPLATETM - Bulk Grain Screening Kit
|  |
Intended
Use |
The EnviroLogix QualiplateTM
Cry1Ab Bulk Grain Screening Kit Is Designed For The
Non-Quantitative Laboratory Detection Of Bt-11, MON-810
Or Bt-176 Cry1Ab Endotoxin In Corn Leaf Tissue, Or Bt-11
And MON-810, Only, In Single Corn Seed Samples.
| |
General
Use |
The
EnviroLogix QualiPlateTM Cry1Ab Bulk Screening
Kit is a “sandwich” Enzyme-Linked ImmunoSorbent Assay
(ELISA). In the test, plant leaf or seed sample extracts
are added to test wells coated with antibodies raised
against Cry1Ab toxin. Any residues present in the sample
extract bind to the antibodies, and are then detected
by addition of enzyme (horseradish peroxidase) labeled
Cry1Ab antibody. After a simple wash step, the results
of the assay are visualized with a color. Development
step; color development is proportional to Cry1Ab concentration
in the sample extract.
Lighter
color = Lower concentration
Darker
color = Higher concentration
| Materials
Provided: |
Materials Required
But Not Provided: |
|
1 x |
Cry1Ab
Antibody-Coated Solid Plate.
|
|
1 x |
Cry1Ab
Positive Control (1 ppb Cry1Ab). |
|
1 x |
Cry1Ab-Enzyme
Conjugate.
|
|
1 x |
Substrate. |
|
|
PBS/0.05%
Tween-20 Wash Buffer.
|
|
|
Milk
Extraction Buffer - PBS/0.55% Tween-20/0.4%
non-fat dry milk. |
|
1 N Hydrochloric acid (HCl) Stop Solution. |
|
|
Disposable
Tip, Adjustable Air-Displacement Pipettes
which will measure 100 microliters (µL),
preferably of multi-channel style. |
|
Marking
Pen (indelible). |
|
|
Tape
or Parafilm®. |
|
Timer. |
|
|
Microtiter
Plate Reader. |
|
Wash Bottle, or microtiter plate or strip
washer. |
|
|
Orbital Plate Shaker (optional). |
|
| Kit
Specifications: |
| Format: |
-
Antibody Coated Microwell Plate (solid plate).
|
| Time
Requirement: |
|
| Detection
Limit: |
- Will
detect one kernel in 800.
|
| Measurement: |
- Microtiter
Plate Reader (450 nm).
|
| Product
ID #: |
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