| ENVIROLOGIX
Inc. – GMO Monitoring Kits
LIBERTY
LINK® Pat/bar Plate Kit (ELISA Kit)
|  |
Intended
Use |
The
EnviroLogix Libertylink® PAT/Bar Plate
Kit Is Designed For The Qualitative Laboratory Detection
Of Phosphinothricin Acetyl Transferase Enzyme (PAT)
Coded For By The Bar Gene In Grain, Leaf, Or
Seed. Two Assay Protocols Are Presented: The High Sensitivity
Protocol Will Detect The PAT Enzyme From The Bar
Gene (PAT/Bar) Found In 0.1% Starlink Corn
(By Weight) And Requires 2.5 Hours To Run. The Rapid
Protocol (One Hour Total) Is Intended For Use In Screening
Individual Seeds Or Leaf Samples For The Presence Of
PAT/Bar. Libertylink PAT From The Pat Gene,
At Concentrations Present In T25 Corn, Is Not Detected
In Either Format.
| |
General
Use |
|
The EnviroLogix
LibertyLink® PAT/bar Plate
Kit is a “sandwich” Enzyme-Linked ImmunoSorbent Assay
(ELISA). In the test, corn or cotton sample
extracts are added to test wells coated with antibodies
raised against PAT from the bar gene. Any PAT/bar
present in the sample extract binds to the antibodies,
and is then detected by addition of enzyme (horseradish
peroxidase).labeled PAT/bar antibody. After
a simple wash step, the results of the assay are visualized
with a color development step; color development is
proportional to PAT/bar concentration in the
sample extract.
Lighter
color = Lower concentration
Darker
color = Higher concentration
| Materials
Provided: |
Materials Required
But Not Provided: |
|
1 x |
Antibody-Coated
Solid Plate. |
|
1 x |
Bottle
Of PAT/bar-Enzyme Conjugate. |
|
1 x |
Packet
Of Wash/Extraction Buffer Salts. |
|
1 x |
Bottle
Of Substrate. |
|
1 x |
Bottle
Of Stop Solution. |
|
|
Distilled
Or Deionized water for preparing Wash/Extraction
Buffer. |
|
|
Glass Bottles or flask plus graduated cylinder
with 1 liter capacity for preparation and
storage of Wash/Extraction Buffer. |
|
Test
Or Centrifuge Tubes for extraction of grain. |
|
|
Snap-Cap
Tubes And Pestles for extraction of leaf samples
(EnviroLogix Cat# ACC 002, 100/package). |
|
Centrifuge
capable of 5000 x g. |
|
|
Disposable Tip, Adjustable Air Displacement
Pipettes which will measure 50 and 100 microliters
(µL). |
|
Marking
Pen (indelible). |
|
|
Tape
or Parafilm. |
|
Timer. |
|
|
Microtiter Plate Reader or strip reader. |
|
Wash
Bottle, or microtiter plate or strip washer. |
|
|
Multi-Channel
Pipette that will measure 50 and 100 mL (optional). |
|
Racked
Dilution Tubes for loading samples into the
plate with a multi-channel pipette (optional). |
|
|
Orbital
Plate Shaker (optional). |
|
| Kit
Specifications: |
| Format: |
- Antibody-Coated
Solid Plate.
|
| Time
Requirement: |
-
High Sensitivity Protocol: 2.5 h.
-
Rapid Protocol: 1 h.
|
| Detection
Limit: |
- High
Sensitivity Protocol: 0.1% Starlink Corn Grain.
-
Rapid Protocol: qualitative (yes/no).
|
| Measurement: |
-
Microtiter Plate Reader or strip reader.
|
| Product
ID #: |
|
|
