ENVIROLOGIX
Inc. - GMO Monitoring Kits
LIBERTY
LINK® Pat/pat Plate Kit (ELISA Kit)
|  |
Intended
Use |
The EnviroLogix
Libertylink® PAT/Pat
Plate Kit Is Designed For The Qualitative Laboratory
Detection Of Phosphinothricin Acetyl Transferase Enzyme
(PAT) Coded For By The Pat Gene In Grain, Leaf,
Or Seed. Two Assay Protocols Are Presented. The High
Sensitivity Protocol Will Detect The PAT Enzyme From
The Pat Gene (PAT/Pat) Found In 0.2% T25
Corn And 3% Bt11 Corn (By Weight) And Requires 2.5 Hours
To Run. The Rapid Protocol (One Hour Total) Is Intended
For Use In Screening Individual Seeds Or Leaf Samples
For The Presence Of PAT/Pat. Libertylink PAT
From The Bar Gene, At Concentrations Present
In Starlink® Corn, Is Not Detected In Either Format.
| |
General
Use |
|
The
EnviroLogix LibertyLink®
PAT/pat Plate Kit is a “sandwich” Enzyme-Linked
ImmunoSorbent Assay (ELISA). In the test, corn or
cotton sample extracts are added to test wells
coated with antibodies raised against PAT from the pat
gene. Any residues present in the sample extract
bind to the antibodies, and are then detected by addition
of enzyme (horseradish peroxidase) labeled PAT/pat
antibody. After a simple wash step, the results
of the assay are visualized with a color development
step; color development is proportional to PAT/pat
concentration in the sample extract.
Lighter
color = Lower concentration
Darker
color = Higher concentration
| Materials
Provided: |
Materials Required
But Not Provided: |
|
1 x |
Antibody-Coated
Solid Plate. |
|
1 x |
Bottle
Of Enzyme Conjugate |
|
1 x |
Packet
Of Wash/Extraction Buffer Salts. |
|
1 x |
Bottle
Of Substrate. |
|
1 x |
Bottle
Of Stop Solution. |
|
|
Distilled
Or Deionized water for preparing Wash/Extraction
Buffer. |
| |
Glass Bottles or flask plus graduated cylinder
with 1 liter capacity for preparation and
storage of Wash/Extraction Buffer. |
|
Test
Or Centrifuge Tubes for extraction of grain. |
| |
Snap-Cap
Tubes And Pestles for extraction of leaf samples
(EnviroLogix Cat# ACC 002, 100/package). |
|
Centrifuge
capable of 5000 x g. |
| |
Disposable Tip, Adjustable Air Displacement
Pipettes which will measure 50 and 100 microliters
(µL). |
|
Marking
Pen (indelible). |
| |
Tape
or Parafilm. |
|
Timer. |
| |
Microtiter Plate Reader or strip reader. |
|
Wash
Bottle, or microtiter plate or strip washer. |
| |
Multi-Channel
Pipette that will measure 50 and 100 mL (optional). |
|
Racked
Dilution Tubes for loading samples into the
plate with a multi-channel pipette (optional). |
| |
Orbital
Plate Shaker (optional). |
|
| Kit
Specifications: |
| Format: |
- Antibody-Coated
Solid Plate.
|
| Time
Requirement: |
-
High Sensitivity Protocol: 3 h 15 min.
-
Rapid Protocol: 1 h 45 min.
|
| Detection
Limit: |
- High
Sensitivity Protocol: 0.2%.
-
Rapid Protocol: qualitative.
|
| Measurement: |
-
Microtiter Plate Reader or strip reader.
|
| Product
ID #: |
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