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R-BIOPHARM - RIDASCREEN®

NITROFURAN (AMOZ) (ELISA Kit)

Intended Use

Competitive Enzyme Immunoassay For The Quantitative Analysis Of AMOZ In Shrimp and Meat (Chicken, Beef, And Pork).

General

Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. They had been also used as growth promoters in the pig production, the poultry and fish sector. In long term studies with experimental animals the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics. This has led to a prohibition of nitrofurans for the treatment of animals used for food production. The nitrofuran drugs furaltadone, nitrofurantoin and nitrofurazone were banned from use in food animal production in the EU in 1993, and the use of furazolidone was prohibited in 1995. The analysis of residues of nitrofuran drugs needs to be based on the detection of the tissue bound metabolites of the nitrofuran parent drugs. Since the parent drugs are very rapidly metabolized, they are not detectable shortly after treatment. The tissue bound nitrofuran metabolites are detectable for a long time after administration and therefore they are used for the detection of the abuse of nitrofurans. Nitrofuran metabolites are found after administration of Furazolidone (metabolite: 3-amino-2-oxazolidinone = AOZ), Furaltadone (metabolite: 3-amino-5- morpholinomethyl-2-oxazolidinone = AMOZ), Nitrofurantoin (metabolite: 1- aminohydantoin = AHD) and Nitrofurazone (metabolite: semicarbazide = SEM).

AMOZ-residues are determined most commonly by LC-UV, LC-MS, or LC-MS/MS techniques. Enzyme immunoassays, compared with chromatographic methods, show considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement.
Using the RIDASCREEN ® Nitrofuran (AMOZ) test, it is possible to detect AMOZ in shrimps, meat (chicken, pork, beef) and milk with little preparation of the sample.

Materials Provided: Materials Required But Not Provided:
  • 1 x
    		•  Microtiter Plate (12 strips with 8 removable wells each) coated with capture antibodies to anti-AMOZ antibodies.
  • 6 x
    		•  Standard Solutions (1.3 ml each) 0 ppb (zero standard), 100 ppt, 300 ppt, 900 ppt, 2700 ppt, 8100 ppt AMOZ in Aqueous Solution.
  • 1 x

    • Conjugate (6 ml); peroxidase conjugated AMOZ; red cap.
  • 1 x
    		•  Anti-AMOZ Antibody (6 ml); black cap.
  • 1 x
    		•  Subtrate/Chromogen (10 ml); blue cap; contains tetramethylbenzidine.
  • 1 x
    		•  Stop Reagent (14 ml); contains 1 N sulfuric acid; yellow cap.
  • 1 x
    		•  Sample and Washing Buffer (salt); for preparation of a 10 mM Phosphate Buffer; pH 7.4 contains 0.05 % Tween 20.
  		• Microtiter Plate Spectrophotometer (450 nm).
  		• Centrifuge.
  		• Ultra-Turrax or Mixer.
  		• Rotary Evaporator.
  		• Shaker (Vortex).
  		• Magnetic Stirrer.
  		• Pasteur Pipettes.
  		• Graduated Pipettes.
  		• Adjustable Micropipettes: 50 µl, 100 µl and 1000 µl.

    Kit Specifications:
    Plate Format: 
    • 96 Wells.
    Time Requirement: 
    • 1 h 30 min.
    Detection Limit:
    • Approx. 200 ppt.
    Sample Preparation:    
    • Homogenization, Derivation, Extraction, Centrifugation, Concentration and Degreasing.
    Recovery Rate:
    • Shrimp 90 - 100 %.
    • Meat 70 - 80 %.
    Cross Reaction:
    • AOZ < 0.05 %.
    • AHD < 0.05 %.
    • SEM < 0.05 %.
    Samples Per Kit:
    • Up to 42.
    Standards:
    • 0/100/300/900/2700 /8100 ppt.
    Measurement: 
    • Microtiter Plate Reader (450 nm).
    Product ID #:
    • R3711.