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ENVIROLOGIX Inc.  - Environmental & Food Monitoring Kits:  Pesticides

CYCLODIENES  (ELISA Kit)

 Intended Use

The EnviroLogix Cyclodiene Plate Kit Is Designed For The Quantitative Laboratory Detection Of Cyclodiene Pesticide Residues In Ground And Surface Water Samples, With An Assay Range From 1 To 100 Parts Per Billion (Ppb).

General

 

The EnviroLogix Cyclodiene Plate Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, Cyclodiene pesticide residues in the sample compete with enzyme (horseradish peroxidase) labeled Chlordane for a limited number of antibody binding sites on the inside surface of the test wells.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker Color = Lower Concentration

Lighter Color = Higher Concentration

The EnviroLogix Cyclodiene Plate Kit can be used in the following applications:

  • Source Water Protection.
  • Treated Effluent Monitoring.
  • Drinking Water Monitoring.
  • Run-Off Assessment & Monitoring.
  • Point Source Testing.
  • Soil Testing.

Materials Provided: Materials Required But Not Provided:
  • 8 x
    		•  Strips of 8 Antibody-Coated Wells each, in plate frame.
  • 1 x
    		•  Vial of 1 ppb Chlordane Calibrator, 100 x Stock Solution (100 ppb in methanol).
  • 1 x
    		• Vial of 10 ppb Chlordane Calibrator, 100 x Stock Solution (1 part per million (ppm) in methanol).
  • 1 x
    		•  Vial of 100 ppb Chlordane Calibrator, 100 x Stock Solution (10 ppm in methanol).
  • 1 x
    		• Bottle of Cyclodiene Sample Dilutent.
  • 1 x
    		•  Bottle of Chlordane-enzyme Conjugate.
  • 1 x
    		•  Bottle of Substrate.
  • 1 x
    		•  Bottle of Stop Solution.
    • Distilled Water (or equivalent) for preparation of calibrators
    • Glass Vials or tubes for preparation of calibrators
    • Positive-Displacement Pipettor with disposable tips and plungers for preparation of calibrators (capable of delivering 50 microliters (µL)).
    • Pipette capable of delivering 5 mL.
    • Disposable Tip, Adjustable Air Displacement Pipette which will measure 50 and 100 µL.
    • Marking Pen (indelible).
    • Tape or Parafilm.
    • Timer (30 minutes).
    • Cool Tap or Distilled Water for rinsing wells.
    • Microtiter Plate Reader or strip reader.
    • Microtiter Plate Washer (optional).
    • Multi-Channel Pipette that will measure 50 µL and 100 µL (optional).
    • Racked Dilution Tubes for loading samples into the plate with a multichannel pipette (optional).
    • Orbital Plate Shaker (optional).

    Kit Specifications:
    Plate Format: 
    • 96 Well.
    Time Requirement: 
    • 2 h 15 min.
    Detection Limit:
    • 0.5 ppb.
    Quantification Limit:     
    • 2 ppb.
    Cross Reaction:
    • Chlordane (0.5 ppb).
    • Aldrin (0.1 ppb).
    • Dieldrin (0.1 ppb).
    • Endosulfan I (0.1 ppb).
    • Endosulfan II (0.1 ppb).
    • Endrin (0.05 ppb).
    • Heptachlor (0.08 ppb).
    • Lindane (5 ppb).
    • Toxaphene (13 ppb).
    Measurement: 
    • Microtiter Plate Reader or Strip Reader
    Product ID #:
    • EP010.

     
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