| ENVIROLOGIX
Inc. - Human Exposure Monitoring Kits
DEET
(ELISA Kit)
|  |
Intended Use
|
The
EnviroLogix Deet In Urine Plate Kit Is Designed For
The Quantitative Laboratory Detection Of Deet Insect
Repellent Residues In Urine Samples, With An Assay Range
From 15 To 750 Parts Per Billion (Ppb).
| |
General |
|
The
EnviroLogix Deet in Urine Plate Kit is a competitive
Enzyme-Linked ImmunoSorbent Assay (ELISA). In the test,
Deet insect repellent residues in the sample compete
with enzyme (horseradish peroxidase)-labeled Deet for
a limited number of antibody binding sites on the inside
surface of the test wells. After a simple wash step,
the outcome of the competition is visualized with a
color development step. As with all competitive immunoassays,
sample concentration is inversely proportional to color
development.
Darker
color = Lower concentration
Lighter
color = Higher concentration
The
EnviroLogix Deet in Urine Plate Kit can be used in the
following testing applications:
- Human
Exposure Monitoring (Applicators, Handlers, Users).
- Public
Health Screening Programs.
- Health
Risk Assessment.
| Materials
Provided: |
Materials Required
But Not Provided: |
|
|
8
x |
|
Strips
Of 12 Antibody-Coated Wells each, in plate
frame. |
|
|
1
x |
|
Vial of 15 ppb Deet Calibrator Stock Solution
(50X in methanol, Actual Concentration = 0.3
ppb*). |
|
|
1
x |
|
Vial
of 100 ppb Deet Calibrator Stock Solution
(50X in methanol, Actual Concentration = 2
ppb*). |
|
|
1
x |
|
Vial
of 750 ppb Deet Calibrator Stock Solution
(50X in methanol, Actual Concentration = 15
ppb*). |
|
|
1
x |
|
Bottle
of 10x Sample Diluent |
|
|
1
x |
|
Bottle
of Deet-enzyme Conjugate. |
|
|
1
x |
|
Bottle
of Substrate. |
|
|
1
x |
|
Bottle
of Stop Solution |
| |
|
* |
The difference between the stated and actual
concentration is to correct for the 1:50 dilution
required for sample preparation |
|
|
Distilled
Water (or equivalent) for preparation of calibrators
and dilution of sample diluent concentrate.
|
|
|
Glass
Vials or tubes for dilution of calibrators
and urine samples. |
|
Positive-Displacement
Pipetter with disposable tips and plungers
for preparation of calibrators (capable of
delivering 20 microliters (µL)). |
|
|
Pipette(s)
capable of delivering 4.9 mL. |
|
Disposable
Tip, Adjustable Air Displacement Pipette which
will measure 100 µL. |
|
|
200
ml graduated cylinder. |
|
Marking Pen (indelible). |
|
|
Tape or Parafilm. |
|
Timer
(1 hour and 30 minutes). |
|
|
Cool
Tap Or Distilled Water for rinsing wells.
|
|
Microtiter
Plate Reader or strip reader. |
|
|
Microtiter
Plate Washer (optional). |
|
Twelve-Channel
Pipette that will measure 100 µL (optional). |
|
|
Racked
Dilution Tubes for loading samples into the
plate with a 12-channel pipette (optional). |
|
|
Orbital
Plate Shaker (optional).
|
|
| Kit
Specifications: |
| Plate
Format: |
|
| Time
Requirement: |
|
| Detection
Limit: |
|
| Quantification
Limit: |
|
Cross
Reaction:
(Limit
Of Detection) |
- Deet
(N,Ndiethyl-m-Toluamide) (0.2 ppb).
- o-Toluamide
(>10000 ppb).
-
m-Toluamide (>10000 ppb).
- p-Toluamide
(>10000 ppb).
- Caffeine
(>10000 ppb).
|
| Measurement: |
-
Microtiter Plate Reader or Strip Reader.
|
| Product
ID #: |
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