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ENVIROLOGIX Inc. - Environmental & Food Monitoring Kits:  Pesticides

METHOPRENE ACID  (ELISA Kit)

 Intended Use 

The EnviroLogix Methoprene Acid Plate Kit Is Designed For The Quantitative Laboratory Detection Of Methoprene Acid Pesticide Residues In Ground And Surface Water Samples, With A Quantitative Assay Range From 0.015 To 1.0 Parts Per Billion (Ppb).

General

 

The EnviroLogix Methoprene Acid Plate Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, Methoprene Acid pesticide residues in the sample compete with enzyme (horseradish peroxidase) labeled Methoprene Acid for a limited number of antibody binding sites on the inside surface of the test wells.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker color = Lower concentration

Lighter color = Higher concentration

The EnviroLogix Methoprene Acid Plate Kit can be used in the following applications:

  • Source Water Protection.
  • Drinking Water Monitoring.
  • Treated Effluent Monitoring.
  • Run-Off Assessment And Monitoring.
  • Point Source Testing.
  • Soil Testing.

Materials Provided: Materials Required But Not Provided:
  •  
    		• 8 x   Strips of 12 Antibody-Coated Wells each, in plate frame.
  •  
    		•  1 x   Vial of 0.015 ppb Methoprene Acid Calibrator (100X in methanol).
  •  
    		• 1 x   Vial of 0.15 ppb Methoprene Acid Calibrator (100X in methanol).
  •  
    		• 1 x   Vial of 1.0 ppb Methoprene Acid Calibrator (100X in methanol).
  		• 10 x   Assay Diluent Concentrate.
  •  
    		• 1 x   Bottle of Methoprene Acid-Enzyme Conjugate.
  •  
    		• 1 x   Bottle of Substrate.
  •  
    		• 1 x   Bottle of Substrate.

  • Distilled Water (or equivalent) for dilution of assay diluent concentrate.
  		• Glass Vials or tubes for preparation of calibrators.
  		• Glass or Plastic Bottle for the storage of 1 x assay diluent.
  		• Graduated Cylinder that can measure 270 mL.
  		• Positive-Displacement Pipettor with disposable tips and plungers for preparation of calibrators (capable of delivering 20 microliters (µL)).
  		• Pipette(s) capable of delivering 2.0 mL.
  		• Disposable Tip, Adjustable Air Displacement pipette which will measure 100 µL.
  		• Marking Pen (indelible).
  		• Tape or Parafilm.
  		• Timer (15 minutes, 1 hour and 30 minutes).
  		• Cool tap or distilled water for rinsing wells.
  		• Microtiter Plate Reader or strip reader.
  		• Microtiter Plate Washer (optional).
  		• Twelve-Channel Pipette that will measure 100 µL (optional).
  		• Racked Dilution Tubes for loading samples into the plate with a 12-channel pipette (optional).
  		• Orbital Plate Shaker (optional).

    Kit Specifications:
    Plate Format: 
    • 96 Well.
    Time Requirement: 
    • 2 h 30 min.
    Detection Limit:
    • 0.007 ppb (water).
    Quantification Limit:     
    • 0.025 ppb.
    Cross Reaction:
    • Methoprene Acid (0.007 ppb).
    • S-Methoprene (0.5 ppb).
    • RS-Methoprene (0.2 ppb).
    • S-Hydroprene (847 ppb).
    • S-Kinoprene (>1000 ppb).
    • Cis-Methoprene (1.6 ppb).
    • Kabat (0.5 ppb).
    • Dianex (0.533 ppb).
    Measurement: 
    • Microtiter Plate Reader or Strip Reader.
    Product ID #:
    • EP016.