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ENVIROLOGIX Inc. - Environmental & Food Monitoring Kits: Pesticides

PARATHION (ELISA Kit)

Intended Use  

The EnviroLogix Parathion Plate Kit Is Designed For The Quantitative Laboratory Detection Of Parathion Pesticide Residues In Ground And Surface Water Samples, With An Assay Range From 0.04 To 4 Parts Per Billion (Ppb).

General

The EnviroLogix Parathion Plate Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, Parathion pesticide residues in the sample compete with enzyme (horseradish peroxidase) labeled Parathion for a limited number of antibody binding sites on the inside surface of the test wells.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker color = Lower concentration

Lighter color = Higher concentration

The EnviroLogix Parathion Plate Kit can be used in the following applications:

  • Source Water Protection.
  • Drinking Water Monitoring.
  • Treated Effluent Monitoring.
  • Run-Off Assessment And Monitoring.
  • Crop And Food Testing.
  • Soil Testing.

Materials Provided: Materials Required But Not Provided:
  • 8 x
    		•  Strips of 12 Antibody-Coated Wells each, in plate frame.
  • 1 x
    		•  Vial of 1000 ppb Parathion Calibrator Stock Solution (in methanol).
  • 1 x
    		• Bottle of Parathion-enzyme conjugate.
  • 1 x
    		•  Bottle of Substrate.
  • 1 x
    		• Bottle of Stop Solution.
  		• Distilled Water (or equivalent) for preparation of calibrators.
  		• Glass Vials or tubes for preparation of calibrators (do not prepare or store calibrators in any plastic containers).
  		• Positive-Displacement Pipetter with disposable tips and plungers for preparation of calibrators (capable of delivering 20 microliters (µL)).
  		• Pipette(s) capable of delivering 5, 4.95, 3.5, 0.5 and 0.05 mL.
  		• Disposable Tip, Adjustable Air Displacement Pipette which will measure 100 µL.
  		• Marking Pen (indelible).
  		• Tape or Parafilm.
  		• Timer (30 minutes).
  		• Cool Tap Or Distilled Water for rinsing wells.
  		• Micro Titer Plate Reader or strip reader.
  		• Microtiter Plate Washer (optional).
  		• Twelve-Channel Pipette that will measure 100 µL (optional).
  		• Racked Dilution Tubes for loading samples into the plate with a 12-channel pipette (optional).
  		• Orbital Plate Shaker (optional).

    Kit Specifications:
    Plate Format: 
    • 96 Well.
    Time Requirement: 
    • 2 h 15 Min.
    Detection Limit:
    • 0.023 ppb.
    Quantification Limit:     
    • 0.06 ppb.
    Cross Reaction:
    • Parathion (0.023 ppb).
    • Coumaphos (0.013 ppb).
    • Quinalphos (0.4 ppb).
    Measurement: 
    • Microtiter Plate Reader or Strip Reader.
    Product ID #:
    • EP025.