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ENVIROLOGIX Inc. - Environmental & Food Monitoring Kits: Pesticides

SYNTHETIC PYRETHOIDS (ELISA Kit)

  Intended Use

This Kit Is Designed To Quantitate Synthetic Pyrethroid Residues In Methanol Extracts Of Various Samples.  The Range Of The Assay Is From 4 To 80 Parts Per Billion (Ppb) Of Cyfluthrin In Methanol Extracts.  These Cyfluthrin Calibrators May Be Used To Quantitate Cyfluthrin, And To Approximate Deltamethrin, Cypermethrin, And Lambda-Cyhalothrin Residues, As The Assay Cross-Reactivity For These Compounds Is Nearly Equal.  To Assay Quantitatively For Other Synthetic Pyrethroids, Contact Xygen Diagnostics For Alternative Calibrator Materials.

General

 

The EnviroLogix Synthetic Pyrethroid Plate Kit is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA).  In the test, synthetic pyrethroid pesticide residues in the sample compete with enzyme horseradish peroxidase)-labeled cypermethrin for a limited number of antibody binding sites on the inside surface of the test wells.  After a simple wash step, the outcome of the competition is visualized with a color development step.  As with all competitive immunoassays, sample concentration is inversely proportional to color development.

Darker color = Lower concentration

Lighter color = Higher concentration

The EnviroLogix Synthetic Pyrethoid Plate Kit can be used in the following applications:

  • Grain Storage Protection.
  • Drinking Water Monitoring.
  • Treated Effluent Monitoring.
  • Run-Off Assessment And Monitoring.
  • Source Water Protection.
  • Treatment Chemical Efficacy.

    • Materials Provided: Materials Required But Not Provided:
  • 8 x
    		•  Strips of 12 Antibody-Coated Wells each, in plate frame.
  • 1 x
    		•  Vial of Synthetic Pyrethroid Negative Control (neat methanol).
  • 1 x
    		• Vial of 4 ppb Cyfluthrin Calibrator, in methanol.
  • 1 x
    		•  Vial of 20 ppb Cyfluthrin Calibrator, in methanol.
  • 1 x
    		• Vial of 80 ppb Cyfluthrin Calibrator, in methanol.
  • 1 x
    		•  Vial of Sodium Hydroxide Solution (0.2 N).
  • 1 x
    		•  Bottle of Cypermethrin-enzyme Conjugate.
  • 1 x
    		•  Bottle of Substrate.
  • 1 x
    		•  Bottle of Stop Solution.
  		• Methanol, ACS Reagent Grade for sample extraction.
  		• Distilled Or Deionized Water for calibrator and sample extract dilution.
  		• Glass Vials or test tubes for preparing dilutions.
  		• Positive-Displacement Pipettor with disposable tips and plungers capable of delivering 100 microliters (µL) and 1 milliliter (mL).
  		• Repeating Pipettor, with syringe tip capable of delivering 25 µL and 2 mL (also 3 syringe tips for delivering 100 µL if you use the repeater to deliver conjugate, substrate and stop in the assay).
  		• Vortex Mixer.
  		• Disposable Tip, Adjustable Air Displacement Pipette which will measure 100 µL.
  		• Marking Pen.
  		• Tape or Parafilm.
  		• Timer.
  		• Cool Tap Or Distilled Water for rinsing wells.
  		• Microtiter Plate Reader or strip reader.
  		• Microtiter Plate Washer (optional).
  		• Twelve-Channel Pipette that will deliver 100 µL (if more than 3 strips are run per assay).
  		• Racked Dilution Tubes for loading samples into the plate with a 12-channel pipette (see previous statement).
  		• Orbital Plate Shaker (optional).

    Kit Specifications:
    Plate Format: 
    • 96 Well
    Time Requirement: 
    • 2 h 10 min.
    Detection Limit:
    • 2.5 ppb.
    Quantification Limit:     
    • 4 ppb.
    Cross Reaction:
    (Limit Of Detection)
    • Cyfluthrin (2.5 ppb).
    • Deltamethrin (1.0 ppb).
    • Cypermethrin (1.7 ppb).
    • Lambda-Cyhalothrin (0.8 ppb).
    Measurement: 
    • Microtiter Plate Reader or Strip Reader.
    Product ID #:
    • EP012.