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Glossary

DON: Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. Of the trichothecene mycotoxins deoxynivalenol, 3-acetyl- and 15-acetyl-deoxynivalenol are the toxins most frequently occurring in Europe and North America. Due to their high cytotoxic and immunosupressive properties these toxins pose a risk to human and animal health.
ELISA: An acronym for Enzyme Linked Immuno Sorbent Assay. The basis of this test is the antigen-antibody reaction. The attribute being tested for competes with other antibodies for binding sites in microwells. After a timed incubation period, the microwells are washed out, chromagen is added which will react colour-wise with the other antibodies. A specific period of time is allowed for colour development, a stop solution is added and the optical density of each well is read by a spectrophotometer. In most cases a multiple regression analysis is run on the results to determine actual values in the standards and samples.
FAST: Describes an ELISA kit format that modifies standard ELISA incubation times to reduce the time required to achieve reliable, quantitative test results.
GFAP: Glial Fibrillary Acidic Protein is a cellular marker, which is highly concentrated in CNS (central nervous system tissue) and very specific for this tissue. CNS such as brain and spinal cord is considered to be the main carrier of BSE. Therefore it is important that his tissue is excluded from the human food chain and is monitored in the form of final product control.
PCR: Polymerase Chain Reaction is an artificial replication system based on numerous replication cycles (30-40) where DNA is doubled constantly.
QUICK: This prefix describes a test format, often employing Colorimetric technology that quickly and easily provides a qualitative test result.